Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

15 July 2002

Biophysical characterization of albumin preparations from blood serum of healthy donors and patients with renal diseases. Part I: Spectrofluorometric analysis

Tatiana V. Parkhomenko, Oksana A. Klicenko, Mikhail M. Shavlovski, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin T. Turoverov

Med Sci Monit 2002; 8(7): BR261-265 :: ID: 13615


Background: Serum albumin (SA) is a major proteinacous component of the blood plasma. In the present study we report on the determination and structural characterization of the modified SA in
the plasma of glomerulonephritis (GN) patients. Fluorescence analysis of albumin-enriched fractions (AEF) isolated from serum of GN patients leads to the discovery of a proteinacous component with essential blue shift of intrinsic fluorescence.
Material/Methods: AEF were isolated from serum blood of 35 GN patients, 30 GN patients with chronic renal failure (CRF) corrected by hemodialysis (HD) and 40 healthy donors. Their fluorescence characteristics have been compared using variations in the value of parameter A = I320/I365 that characterizes fluorescence spectrum position and shape.
Results: The fluorescence spectra of AEF from GN and GN with CRF patients were blue- and redshifted, respectively, in comparison with spectra of donors’ serum. The value of parameter A was 1.27±0.05 for protein preparations from healthy donors, while this characteristic lies
within range of 1.3–2.1 or 0.77–1.29 for GN and GN with CRF patients, respectively. Moreover, for GN patients, the magnitude of spectral parameter A was approaching control values during the treatment of disease.
Conclusion: hSA enriched fractions isolated from GN patients or GN patients with CRF corrected by HD differ by their spectrofluorometric properties from those of healthy donors. This technique allows the detection of renal diseases and the efficiency of their cure.

Keywords: Glomerulonephritis - blood, Glomerulonephritis - therapy, Kidney Failure, Chronic - blood, Kidney Failure, Chronic - therapy, Serum Albumin - chemistry, Serum Albumin - metabolism, Spectrometry, Fluorescence, Statistics

Add Comment 0 Comments


01 June 2024 : Editorial  

Editorial: Concerns as Highly Pathogenic Avian Influenza (HPAI) Virus of the H5N1 Subtype is Identified in Dairy Cows and Other Mammals

Dinah V. Parums

DOI: 10.12659/MSM.945315

Med Sci Monit 2024; 30:e945315


In Press

Review article  

Applications of Functional Near-Infrared Spectroscopy (fNIRS) Neuroimaging During Rehabilitation Following ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943785  


Clinical Research  

Epidemiology and Risk Factors for Revision Total Knee Arthroplasty in Chinese Patients: A Retrospective Stu...

Med Sci Monit In Press; DOI: 10.12659/MSM.943681  

Clinical Research  

Effects of the Cold Pressor Test on Popliteal Vein Diameter, Flow Velocity, and Blood Flow in the Lower Lim...

Med Sci Monit In Press; DOI: 10.12659/MSM.944560  

Clinical Research  

Outcomes of Anterior Lateral Approach and Gerdy's Tubercle Osteotomy for Tibial Plateau Fractures Involving...

Med Sci Monit In Press; DOI: 10.12659/MSM.943946  

Most Viewed Current Articles

14 Dec 2022 : Clinical Research   1,477,232

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990


17 Jan 2024 : Review article   1,433,627

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799


16 May 2023 : Clinical Research   690,157

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387


01 Jan 2022 : Editorial   50,150

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952


Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750