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12 August 2020 : Laboratory Research

[Retracted: 10 Apr 2023] A Regulatory Axis of circ_0008193/miR-1180-3p/TRIM62 Suppresses Proliferation, Migration, Invasion, and Warburg Effect in Lung Adenocarcinoma Cells Under Hypoxia

Minbiao Chen1ABC, Xiuming Huang1CDE, Liang Li1CDE, Mingfang Huang1BCD, Renzhong Cai1ABC, Xuqiang Liao1CDE*

DOI: 10.12659/MSM.922900

Med Sci Monit 2020; 26:e922900

This publication has been retracted by the Editor due to non-original content and deficiencies in the conduct of the study. Reference: Minbiao Chen, Xiuming Huang, Liang Li, Mingfang Huang, Renzhong Cai, Xuqiang Liao.A Regulatory Axis of circ_0008193/miR-1180-3p/TRIM62 Suppresses Proliferation, Migration, Invasion, and Warburg Effect in Lung Adenocarcinoma Cells Under Hypoxia. Med Sci Monit, 2020; 26: e922900. DOI: 10.12659/MSM.922900


BACKGROUND: Expression profiles of circular ribonucleic acids (circRNAs) have been recently reported in lung cancers including lung adenocarcinoma (LUAD). Hypoxia is a hallmark of lung cancers. However, the role of hsa_circ_0008193 (circ_0008193) in LUAD under hypoxia remains to be illuminated.

MATERIAL AND METHODS: Gene expression levels were detected using real-time quantitative polymerase chain reaction and western blotting. Cell proliferation, migration, invasion, and Warburg effect were detected using 3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide assay, transwell assays, special kits, and xenograft experiments. The relationship among circ_0008193, micro (mi)RNA (miR)-1180-3p, and tripartite motif containing 62 (TRIM62) was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation.

RESULTS: Expression of circ_0008193 was downregulated in human LUAD tumor tissues and cell lines (A549 and H1975), accompanied by miR-1180-3p upregulation and TRIM62 downregulation. Moreover, circ_0008193 downregulation was correlated with tumor size and lymph node metastasis. Functionally, circ_0008193 overexpression inhibited cell viability, glucose uptake, lactate production, migration, and invasion, as well as expression of hexokinase II, lactate dehydrogenase A, matrix metalloproteinase 2 (MMP2), and MMP9 in hypoxic LUAD cells in vitro. Furthermore, tumor growth of A549 cells in vivo was also hindered by circ_0008193 overexpression. Mechanically, circ_0008193 regulated TRIM62 expression via sponging miR-1180-3p, and TRIM62 was targeted by miR-1180-3p. Both miR-1180-3p upregulation and TRIM62 downregulation could abolish the suppressive role of circ_0008193 in LUAD cells.

CONCLUSIONS: Upregulating circ_0008193 inhibited LUAD cell proliferation, migration, invasion, and Warburg effect under hypoxia in vitro and in vivo through regulation of the miR-1180-3p/TRIM62 axis.

Keywords: Retracted Publication

Retraction note

Med Sci Monit 2023; 29:e940684     https://medscimonit.com/abstract/index/idArt/940684
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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750